Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer
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چکیده
منابع مشابه
Detection of intratumor heterogeneity by simultaneous multiparameter flow cytometric analysis with enzyme and DNA markers.
We have developed a rapid flow cytometric technique useful in characterizing tumor cell heterogeneity and detecting infre quent aneuploid or tetraploid cells in various human and murine gynecological tumors. With this technique, DNA and enzyme measurements can be determined simultaneously on cell sus pensions obtained either from malignant effusions or from enzymatic digests of both preinvasive...
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BACKGROUND The objectives of this study were (i) to evaluate whether the combined use of Syto 16 and 7-amino-actinomycin-D (7-AAD) allows the detection of sperm apoptosis and (ii) to describe a new multiparameter flow cytometric method to assess simultaneously sperm concentration (SC), viability and apoptosis as well as leukocyte concentration. METHODS Semen samples from 68 patients were eval...
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We monitored the behavior of residual myeloma plasma cells in patients with multiple myeloma after high-dose therapy and autologous or allogeneic transplantation using 3 methods of a flow cytometric technique using 4-color staining, immunofixation, and polymerase chain reaction approaches. We analyzed 17 cases by a relatively simple flow cytometric technique using CD38/CD45/CD19/CD56. Detectabl...
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PURPOSE To explore intratumor heterogeneity in gene expression profiles from patients with cervical cancer. EXPERIMENTAL DESIGN A total of 33 biopsies were obtained from 11 patients, sampling between two and five different areas for each tumor. The extracted RNA was hybridized onto the Affymetrix U133 Plus 2.0 oligonucleotide chip. The variance of expression within a patient (W), between pati...
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ژورنال
عنوان ژورنال: Cytometry
سال: 2000
ISSN: 0196-4763,1097-0320
DOI: 10.1002/(sici)1097-0320(20000201)39:2<96::aid-cyto2>3.0.co;2-x